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IRI was performed through a flank incision after the animals were anesthetized with 2 to 4% isofluorane inhalation. IRI was induced as previously described (23). The kidney was isolated and a microvascular clamp (Roboz, Rockville, MD) was placed on the renal pedicle for 30 min, during which the kidney and clamp were gently placed back into the peritoneum.

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