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Transduced rMSCs were tested for their ability to continue to exhibit GFP fluorescence after expansion by low density plating. As shown in the right hand column of Figure 4, transduced cells went through at least 10.5 cell doublings and increased in number at least 1400 fold. RMSCs transduced with pSIR PGK GFP maintained GFP expression after increasing in number 1697 fold 89, increased the percentage of GFP positive cells in the culture to 92.2%, and had a similar nike air max 90 aanbieding RFI when compared with before low density expansion.

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