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Top of pageMethodsHarvest and culture expansion of murine MSCsFemale C57Bl/6 mice (18 g) were obtained from Charles River Laboratories (Laprairie Co., Quebec, Canada), and these isogenic mice were used as donors and recipients of MSCs to simulate autologous implantation. Mice were killed by nike air max 2017 dames marktplaats CO2 inhalation and bone marrow cells nike air force 1 low animal harvested by flushing femurs and tibias with DMEM supplemented with 10% FBS and 50 U/ml penicillin/streptomycin. Whole marrow was plated in tissue culture dishes, and 5 nike air max 2017 dames marktplaats days nike roshe run heren aanbieding later the nonadherent hematopoietic cells were discarded.

Try not to pull too hard on the metal tabs or they may rip off. I found that out the hard way while trying to separate the cells. Good thing I bought extras.

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